9/1/2023 0 Comments Ihc antibody cross reactivity![]() There are a number of books on the topics by world leaders such as Clive Taylor and David Dabbs, and recently, an ad hoc group published a set of “recommendations” ( 2). Although many groups have enunciated the need, there are no universally accepted guidelines for best practice in antibody-based tests. The influence of antibody-based tests on clinical decisions has led to a number of publications that have highlighted the unmet need for standardization of such assays and development of antibody validation guidelines ( 1– 8). Thus, in the clinic, as well as in the research laboratory, careful accurate validation of antibody reagents is critical for correct results. ![]() ![]() For example, the assessment of estrogen receptor α (ER-α), and human epidermal growth factor receptor 2 (HER2) by IHC in breast cancer patients is the definitive test to determine whether or not a patient will receive therapies that can cost as much as $100,000 per year. In anatomic pathology, IHC serves as a diagnostic, prognostic, and predictive method and IHC readings directly influence the management of patients in the clinical setting. They are also important tools in clinical management with extensive use in both laboratory medicine (ELISA assays and flow cytometry) and anatomic pathology (IHC). Antibodies are among the most commonly used research tools, routinely used for Western blot (WB), immunoprecipitation (IP), enzyme-linked immunosorbent assays (ELISA), quantitative immunofluorescence (QIF), and immunohistochemistry (IHC). ![]()
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